Size, sequence data and RFLP of the ITS amplicons will therefore be valuable markers for the identification of different Prochlorococcus genotypes and for their discrimination from other cyanobacterial relatives with which they often co-exist in oceanic ecosystems. Restriction fragment length polymorphism (RFLP) of the ITS amplicons revealed three subclusters among the HL strains.
Prochlorococcus isolates of the high-light-adapted clade (HL) differed from representatives of the low-light-adapted Glade (LL) by the length of their ITS. A new group-specific primer permitted ITS amplification even with non-axenic isolates of Prochlorococcus and one OMF-'Synechococcus' strain. In spite of large differences in length, sequence and mean DNA base composition, conserved domains important for transcriptional antitermination and folding of the rRNA transcripts were identified in all ITSs. The ITSs of three strains (PCC 9511(T), PCC 6307 and PCC 7001) were sequenced and compared with those available for strains Prochlorococcus MED4 (CCMP 1378) and MIT9313 from genome sequencing projects. pastoris PCC 9511(T), although these four cyanobacteria cluster relatively closely in phylogenetic trees inferred from 165 rRNA gene sequences.
PCR amplicons of the Internal Transcribed Spacer (ITS) of the rrn operon of three axenic OMF (oceanic, marine and freshwater) strains of 'Synechococcus' (wH7803, PCC 7001 and PCC 6307, respectively) differ greatly in length from that of the axenic Prochlorococcus marinus subsp. Laloui W, Palinska KA, Rippka R, Partensky F, de Marsac NT, Herdman M, Iteman IĢ002, Blue Green Alga, Chlorophyll B, community structure, cyanobacteria, Escherichia Coli, Marine Prokaryote, Multiple Evolutionary Origins, North Atlantic Ocean, Pacific Ocean, rcc, Rna Operon, SBR$_\textrmP$hyto, sbr?hyto
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Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-'Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon What is Iteman Iteman is is a software platform for automated psychometric reporting (test and item analysis) with classical test theory.What does that mean It means that the software doesn’t just calculate statistics it automatically builds you a tech report in MS Word, potentially hundreds of pages, with embedded narratives, graphs, and tables like you see here.